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A vector carrying the GFP gene (Green fluorescent protein) as a yeast marker for fermentation processes Scientia Agricola
Gomes,Luiz Humberto; Duarte,Keila Maria Roncato; Andrino,Felipe Gabriel; Giacomelli,Ana Maria Brancalion; Tavares,Flavio Cesar Almeida.
Contaminant yeasts spoil pure culture fermentations and cause great losses in quality and product yields. They can be detected by a variety of methods although none being so efficient for early detection of contaminant yeast cells that appear at low frequency. Pure cultures bearing genetic markers can ease the direct identification of cells and colonies among contaminants. Fast and easy detection are desired and morphological markers would even help the direct visualization of marked pure cultures among contaminants. The GFP gene for green fluorescent protein of Aquorea victoria, proved to be a very efficient marker to visualize transformed cells in mixed populations and tissues. To test this marker in the study of contaminated yeast fermentations, the GFP...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saccharomyces cerevisiae; GFP (green fluorescent protein); Fermentative processes.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000400018
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HYDROGEN PRODUCTION AND PERFORMANCE OF ANAEROBIC FIXED-BED REACTORS USING THREE SUPPORT ARRANGEMENTS FROM CASSAVA STARCH WASTEWATER REA
Torres,Douglas G. B.; Lucas,Shaiane Dal' Maso; Andreani,Cristiane L.; Carvalho,Karina Q. de; Coelho,Silvia R. M.; Gomes,Simone D..
ABSTRACT Fixed-bed reactors have been considered promise alternatives for hydrogen production due to their simple construction and increase in the biomass retention. The purpose of this study was to investigate the biological production of hydrogen in anaerobic fixed-bed reactors with cassava starch wastewater used as substrate. Different support materials and arrangements of fixed-bed were used to evaluate the biological production of hydrogen in anaerobic continuous fixed-bed reactors, with cassava starch wastewater as substrate - recycled low-density polyethylene scraps, in packed bed (R1), recycled low-density polyethylene cylinders, in ordained bed (R3) and bamboo stems, in vertical arrangement (R2 and R4). In R1 the initial pH was adjusted for 6.0,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bio-energy; Fermentative processes; Bamboo; Polyethylene; Agro-industrial effluent.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162017000100160
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